Acute changes in myo‐inositol uptake and 22Na+ flux in murine neuroblastoma cells (N1E‐115) following insulin

FEBS Letters

Larkins, Richard G.¹  Dunlop, Marjorie¹  Dimitriadis, Eva¹ 

[1] University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, 3050 Victoria, Australia

关键词: myo-Inositol uptake;    Na+;    (Na+ + K+)-ATPase;    Insulin;    Pyrithiamine;    (Neuroblastoma clone);   

DOI  :  10.1016/0014-5793(87)80880-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

myo-Inositol uptake was investigated in a murine neuroblastoma clone (N1E-115) to determine the effect of altered Na⁺,K⁺-ATPase activity. The Na⁺ ionophone monensin, and veratridine, an alkaloid affecting voltage-dependent Na⁺ entry, increased acute ²²Na⁺ uptake and ²²Na⁺ efflux from pre-loaded cells, concomitant with enhanced myo-inositol uptake. This effect was also seen following insulin. Insulin-stimulated myo-inositol uptake was inhibited by amiloride, ouabain and pyrithiamine. Amiloride inhibition suggests that activation of Na⁺/H⁺ exchange preceding Na⁺,K⁺-ATPase activation is involved in insulin stimulation of myo-inositol uptake. Pyrithiamine inhibition is an indication of prior activation of the Na⁺,K⁺-ATPase α⁺ catalytic subunit by insulin. The results provide evidence that insulin contributes to the maintenance of Na⁺, K⁺-ATPase in neuronal tissue.

【 授权许可】

Unknown