FEBS Letters | |
Acute changes in myo‐inositol uptake and 22Na+ flux in murine neuroblastoma cells (N1E‐115) following insulin | |
Larkins, Richard G.1  Dunlop, Marjorie1  Dimitriadis, Eva1  | |
[1] University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, 3050 Victoria, Australia | |
关键词: myo-Inositol uptake; Na+; (Na+ + K+)-ATPase; Insulin; Pyrithiamine; (Neuroblastoma clone); | |
DOI : 10.1016/0014-5793(87)80880-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
myo-Inositol uptake was investigated in a murine neuroblastoma clone (N1E-115) to determine the effect of altered Na+,K+-ATPase activity. The Na+ ionophone monensin, and veratridine, an alkaloid affecting voltage-dependent Na+ entry, increased acute 22Na+ uptake and 22Na+ efflux from pre-loaded cells, concomitant with enhanced myo-inositol uptake. This effect was also seen following insulin. Insulin-stimulated myo-inositol uptake was inhibited by amiloride, ouabain and pyrithiamine. Amiloride inhibition suggests that activation of Na+/H+ exchange preceding Na+,K+-ATPase activation is involved in insulin stimulation of myo-inositol uptake. Pyrithiamine inhibition is an indication of prior activation of the Na+,K+-ATPase α+ catalytic subunit by insulin. The results provide evidence that insulin contributes to the maintenance of Na+, K+-ATPase in neuronal tissue.
【 授权许可】
Unknown
【 预 览 】
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