Production of unnatural glucosides of curcumin with drastically enhanced water solubility by cell suspension cultures of Catharanthus roseus

FEBS Letters

Nagatsu, Akito²  Kaminaga, Yasuhisa²  Yamazaki, Takeshi¹  Akiyama, Takumi¹  Mizukami, Hajime²  Sugimoto, Naoki¹  Maitani, Tamio¹ 

[1] National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan;Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori 3-1, Mizuho-ku, Nagoya 467-8603, Japan

关键词: Glucosylation;    Curcumin;    Water solubility;    Cell suspension culture;    Catharanthus roseus;    HMBC;    1H-13C heteronuclear multiple bond connectivity;    MJ;    methyl jasmonate;    SA;    salicylic acid;   

DOI  :  10.1016/S0014-5793(03)01265-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Catharanthus roseus cell suspension cultures converted exogenously supplied curcumin to a series of glucosides, none of which has been found in nature so far. The efficiency of glucosylation was dependent on culture stage of the cells and medium sucrose concentration. Methyl jasmonate and salicylic acid enhanced the glucoside formation only when they were added to the cultures prior to the addition of curcumin. The glucoside yield was 2.5 μmol/g fresh weight of the cells at an optimal culture condition. The water solubility of curcumin-4′,4″-O-β-D-digentiobioside was 0.65 mmol/ml, which was 20 million-fold higher than that of curcumin.

【 授权许可】

Unknown