An early salicylic acid‐, pathogen‐ and elicitor‐inducible tobacco glucosyltransferase: role in compartmentalization of phenolics and H2O2 metabolism

FEBS Letters

Chong, Julie¹  Baltz, Rachel¹  Saindrenan, Patrick¹  Fritig, Bernard¹ 

[1] Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 67000 Strasbourg, France

关键词: Defense response;    Phenylpropanoid;    Hydroxycoumarin;    Glucosylation;    H2O2;    Nicotiana tabacum;    TOGT;    tobacco glucosyltransferase;    HR;    hypersensitive response;    ROIs;    reactive oxygen intermediates;    TMV;    tobacco mosaic virus;    UDPG:scopoletin GTase;    UDP-glucose:scopoletin glucosyltransferase;    DPI;    diphenylene iodonium;   

DOI  :  10.1016/S0014-5793(99)01154-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of tobacco cell suspension cultures with a fungal elicitor of defense responses resulted in an early accumulation of the phenylpropanoid glucosyltransferase TOGT, along with the rapid synthesis and secretion of scopolin, the glucoside of scopoletin. Elicitor-triggered extracellular accumulation of the aglycone scopoletin and of free caffeic and ferulic acids could only be revealed in the presence of diphenylene iodonium, an inhibitor of extracellular H₂O₂ production. Our results strongly support a role for TOGT in the elicitor-stimulated production of transportable phenylpropanoid glucosides, followed by the release of free antioxidant phenolics into the extracellular medium and subsequent H₂O₂ scavenging.

【 授权许可】

Unknown