Evolution of substrate recognition sites (SRSs) in cytochromes P450 from Apiaceae exemplified by the CYP71AJ subfamily

BMC Evolutionary Biology

Research Article

Olivier Taboureau¹  Frédéric Bourgaud²  Alain Hehn²  Célia Krieger²  Alexandre Olry²  Tsunashi Kamo³  Henrik Toft Simonsen⁴  Damian Drew⁴  Corinna Weitzel⁵  Bjørn Dueholm⁶ 

[1] Technical University of Denmark, Centre for Biological and Sequence Analysis (CBS), Anker Engelunds Vej 1, 2800, Kgs. Lyngby, Denmark;Molécules Thérapeutiques in silice (MTi), Inserm UMR-S 973 - Université Paris Diderot, Bat Lamarck A, 35 Rue Hélène Brion, 75205, Paris, France;UMR1121 Université de Lorraine, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;UMR1121 INRA, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;UMR1121 Université de Lorraine, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;UMR1121 INRA, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;National Institute for Agro-Environmental Sciences, 3-1-3 Kan-nondai, 305-8604, Tsukuba, Ibaraki, Japan;University of Copenhagen, Department of Plant and Environmental Science, Copenhagen Plant Science Centre, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark;University of Copenhagen, Department of Plant and Environmental Science, Copenhagen Plant Science Centre, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark;UMR1121 Université de Lorraine, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;UMR1121 INRA, 2 Avenue de la Forêt de Haye, 54518, Vandoeuvre-les-Nancy, France;University of Copenhagen, Department of Plant and Environmental Science, Copenhagen Plant Science Centre, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark;University of South Australia, School of Pharmacy and Medical Sciences, Adelaide, South Australia;

关键词: Cytochrome P450;    Furanocoumarin;    Hydroxycoumarin;    Apiaceae;    Apioideae;    Gene duplication;    Plant-insect coevolution;    CYP71AJ subfamily;    Substrate recognition sites (SRSs);    Pastinaca sativa;   

DOI  :  10.1186/s12862-015-0396-z

 received in 2014-12-13, accepted in 2015-05-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundLarge proliferations of cytochrome P450 encoding genes resulting from gene duplications can be termed as ‘blooms’, providing genetic material for the genesis and evolution of biosynthetic pathways. Furanocoumarins are allelochemicals produced by many of the species in Apiaceaous plants belonging to the Apioideae subfamily of Apiaceae and have been described as being involved in the defence reaction against phytophageous insects.ResultsA bloom in the cytochromes P450 CYP71AJ subfamily has been identified, showing at least 2 clades and 6 subclades within the CYP71AJ subfamily. Two of the subclades were functionally assigned to the biosynthesis of furanocoumarins. Six substrate recognition sites (SRS1-6) important for the enzymatic conversion were investigated in the described cytochromes P450 and display significant variability within the CYP71AJ subfamily. Homology models underline a significant modification of the accession to the iron atom, which might explain the difference of the substrate specificity between the cytochromes P450 restricted to furanocoumarins as substrates and the orphan CYP71AJ.ConclusionTwo subclades functionally assigned to the biosynthesis of furanocoumarins and four other subclades were identified and shown to be part of two distinct clades within the CYP71AJ subfamily. The subclades show significant variability within their substrate recognition sites between the clades, suggesting different biochemical functions and providing insights into the evolution of cytochrome P450 ‘blooms’ in response to environmental pressures.

【 授权许可】

Unknown   
© Dueholm et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.