Investigation of cytokinin‐deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1

FEBS Letters

Yang, Shuhua¹  Yu, Hao¹  Xu, Yifeng¹  Goh, Chong Jin¹ 

[1] Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Science Drive 4, Singapore 117543

关键词: Cytokinin;    Cytokinin oxidase;    DSCKX1;    Shoot/root formation;    Transgenic Arabidopsis;    CIM;    callus-inducing media;    CKX;    cytokinin oxidase;    iPA;    isopentenyladenosine;    ZR;    zeatin riboside;   

DOI  :  10.1016/S0014-5793(03)01259-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased ability to form roots in vitro, and reduced response to cytokinin in growing calli and roots. Furthermore, the expression of KNAT1, STM, and CycD3 genes was significantly reduced in the transgenic plants, suggesting that cytokinin may function to control the cell cycles and shoot/root development via regulation of these genes.

【 授权许可】

Unknown