Monitoring genome‐wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana

FEBS Letters

Chua, Nam-Hai²  Morgante, Michele³  Hanafey, Michael K.³  Tingey, Scott V.³  Gaasterland, Terry¹  Ikeda, Yoshihisa²  Wang, Xiujie¹  Hoth, Stefan²  Zuo, Jianru² 

[1] Laboratory of Computational Genomics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA;Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA;E.I. DuPont de Nemours and Co. (Inc.), DuPont Agriculture and Nutrition – Molecular Genetics, P.O. Box 6104, Newark, DE 19714-6104, USA

关键词: Cytokinin;    Gene expression;    Isopentenyl transferase;    Massively parallel signature sequencing;    IPT;    isopentenyl transferase;    MPSS;    massively parallel signature sequencing;    DEX;    dexamethasone;    MAPK;    mitogen-activated protein kinase;    MAPKKK;    mitogen-activated protein kinase kinase kinase;   

DOI  :  10.1016/S0014-5793(03)01194-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytokinins have been implicated in developmental and growth processes in plants including cell division, chloroplast biogenesis, shoot meristem initiation and senescence. The regulation of these processes requires changes in cytokinin-responsive gene expression. Here, we induced the expression of a bacterial isopentenyl transferase gene, IPT, in transgenic Arabidopsis thaliana seedlings to study the regulation of genome-wide gene expression in response to endogenous cytokinin. Using MPSS (massively parallel signature sequencing) we identified 823 and 917 genes that were up- and downregulated, respectively, following 24 h of IPT induction. When comparing the response to cytokinin after 6 and 24 h, we identified different clusters of genes showing a similar course of regulation. Our study provides researchers with the opportunity to rapidly assess whether genes of interest are regulated by cytokinins.

【 授权许可】

Unknown