Caenorhabditis elegans dna‐2 is involved in DNA repair and is essential for germ‐line development

FEBS Letters

Han, Sung Min²  Koo, Hyeon-Sook²  Han, Ji Won²  Lee, Myon Hee²  Kim, Yun Mi²  Ahnn, Joohong¹ 

[1] Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 506-303, South Korea;Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, South Korea

关键词: dna-2;    Germ-line;    DNA repair;    mre-11;    Caenorhabditis elegans;    Ce-Dna-2;    Caenorhabditis elegans Dna-2;    dsRNA;    double-stranded RNA;    DAPI;    4′;    6′-diamidino-2-phenylindole;    ORF;    open reading frame;    RNAi;    RNA interference;   

DOI  :  10.1016/S0014-5793(03)01243-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Caenorhabditis elegans germ cell proliferation and development were severely damaged in second generation dna-2 homozygotes. Even in the first generation, a much higher incidence of aberrant chromosomes in oocytes and resultantly higher embryonic lethality were found vs. wild type, when DNA breaks were induced by γ-rays or camptothecin. The deficiency of dna-2 in combination with RNA interference on mre-11 gene expression synergistically aggravated germ-line development, especially oocyte formation. These results suggest that C. elegans Dna-2 is involved in a DNA repair pathway paralleling homologous recombination or non-homologous end joining with mre-11 participation.

【 授权许可】

Unknown