A microscope‐based screening platform for large‐scale functional protein analysis in intact cells

FEBS Letters

Wiemann, Stefan²  Liebel, Urban¹  Pepperkok, Rainer¹  Starkuviene, Vytaute¹  Erfle, Holger¹  Poustka, Annemarie²  Simpson, Jeremy C¹ 

[1] Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany;Molecular Genome Analysis, German Cancer Research Centre, Im Neuenheimer Feld 280/506, 69120 Heidelberg, Germany

关键词: Green fluorescent protein;    Proteomics;    Functional analysis;    High-content screening microscopy;    Membrane traffic;    Golgi complex;    CFP;    cyan fluorescent protein;    ER;    endoplasmic reticulum;    GFP;    green fluorescent protein;    ORF;    open reading frame;    YFP;    yellow fluorescent protein;   

DOI  :  10.1016/S0014-5793(03)01197-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A modular microscope-based screening platform, with applications in large-scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome-wide automated retrieval of bioinformatic information. The modular nature of the system ensures that it is rapidly adaptable to new biological questions or sets of proteins. Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects.

【 授权许可】

Unknown