Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease

FEBS Letters

Berisio, R³  Di Fiore, A²  Piccoli, R¹  Zagari, A³  Mazzarella, L³  De Lorenzo, C¹  Sica, F³ 

[1] Dipartimento di Chimica Biologica, Università di Napoli Federico II, Via Mezzocannone 16, 80134 Naples, Italy;Dipartimento di Chimica, Università di Napoli Federico II, Via Cynthia, 80126 Naples, Italy;Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 6, 80134 Naples, Italy

关键词: Three-dimensional domain swapping;    Conformational dimorphism;    Crystal structure;    Ribonuclease;    BS-RNase;    bovine seminal ribonuclease;    MxM;    dimeric form of BS-RNase in which the chains swap their N-terminal helices;    M=M;    unswapped form of BS-RNase;    M;    monomeric BS-RNase;    RNase A;    bovine pancreatic ribonuclease;    hRNase;    human pancreatic ribonuclease;    RI;    ribonuclease inhibitor;    rmsd;    root mean square deviation;   

DOI  :  10.1016/S0014-5793(03)01114-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine seminal ribonuclease is a unique case of protein dimorphism, since it exists in two dimeric forms, with different biological and kinetic behavior, which interconvert into one another through three-dimensional swapping. Here we report the crystal structure, at 2.2 Å resolution, of the unswapped form of bovine seminal ribonuclease. Besides completing the structural definition of bovine seminal ribonuclease conformational dimorphism, this study provides the structural basis to explain the dependence of the enzyme cooperative effects on its swapping state.

【 授权许可】

Unknown