Differential interaction of the two cholesterol‐dependent, membrane‐damaging toxins, streptolysin O and Vibrio cholerae cytolysin, with enantiomeric cholesterol

FEBS Letters

Palmer, Michael²  Westover, Emily J³  Zitzer, Alexander¹  Covey, Douglas F³ 

[1] Department of Hematology and Oncology, Johannes Gutenberg University, D-55101 Mainz, Germany;Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada N2L 3G1;Division of Bioorganic Chemistry, Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA

关键词: Streptolysin O;    Cholesterol-binding cytolysins;    Vibrio cholerae cytolysin;    Protein–cholesterol interaction;    Enantiomeric cholesterol;   

DOI  :  10.1016/S0014-5793(03)01023-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membrane cholesterol is essential to the activity of at least two structurally unrelated families of bacterial pore-forming toxins, represented by streptolysin O (SLO) and Vibrio cholerae cytolysin (VCC), respectively. Here, we report that SLO and VCC differ sharply in their interaction with liposome membranes containing enantiomeric cholesterol (ent-cholesterol). VCC had very low activity with ent-cholesterol, which is in line with a stereospecific mode of interaction of this toxin with cholesterol. In contrast, SLO was only slightly less active with ent-cholesterol than with cholesterol, suggesting a rather limited degree of structural specificity in the toxin–cholesterol interaction.

【 授权许可】

Unknown