The tetanus toxin light chain inhibits exocytosis

FEBS Letters

Dauzenroth, Maria-Elisabeth¹  Gratzl, Manfred²  Ahnert-Hilger, Gudrun²  Weller, Ulrich¹  Habermann, Ernst¹ 

[1] Rudolf-Buchheim-Institut für Pharmakologie der Justus-Liebig-Universität, D-6300 Gießen, FRG;Abteilung Anatomie und Zellbiologie der Universität Ulm, D-7900 Ulm FRG

关键词: Tetanus toxin;    Light chain;    Streptolysin O;    Exocytosis;    (Chromaffin cell);   

DOI  :  10.1016/0014-5793(89)80478-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intracellular action on exocytosis of various forms of tetanus toxin was studied using adrenal medullary chromaffin cells, the membrane barrier of which has been removed by permeabilization with streptolysin O. Such cells still release catecholamines on stimulation with calcium. The two-chain form of tetanus toxin (67 nmol/l) strongly inhibited exocytosis, but only if dithiothreitol was present as a reducing agent. Purified light chain completely prevented [³H]noradrenaline release with a half-maximal effect at about 5 nmol/1. Heavy chain (up to 11 nmol/l) and unprocessed single-chain toxin (up to 133 nmol/l) were without effect. It is concluded that the original single-chain form of tetanus toxin has to be processed by proteolysis and reduction to yield a light chain which inhibits transmitter release.

【 授权许可】

Unknown