Noradrenaline release from permeabilized synaptosomes is inhibited by the light chain of tetanus toxin

FEBS Letters

Stecher, Brigitte¹  Gratzl, Manfred¹  Gispen, Willem-Hendrik³  Hens, Jacques J.H.³  De Graan, Pierre N.E.³  Weller, Ulrich² 

[1] Abteilung Anatomie und Zellbiologie, Universität Ulm, D-7900 Ulm, Germany;Institut für medizinische Mikrobiologie, Universität Mainz, D-6500 Mainz, Germany;Div. Mol. Neurobiology, Rudolf Magnus Institute and Institute of Molecular Biology and Medical Biotechnology, 3584 CH Utrecht, The Netherlands

关键词: Tetanus toxin;    Streptolysin O;    Permeabilization;    Exocytosis;    Synaptosome;    BoNT/A;    botulinum A toxin;    H;    heavy chain;    L;    light chain;    NA;    noradrenaline;    SLO;    streptolysin O;    TeTx;    tetanus toxin;   

DOI  :  10.1016/0014-5793(92)80933-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Noradrenaline release from rat brain cortical synaptosomes permeabilized with streptolysin O can be triggered by μM concentrations of free Ca²⁺. This process was inhibited within minutes by tetanus toxin and its isolated light chain, but not by its heavy chain. The data demonstrate that the effect of tetanus toxin on NA release from purified synaptosomes is caused by the intraterminal action of its light chain.

【 授权许可】

Unknown