Light chain of tetanus toxin intracellularly inhibits acetylcholine release at neuro‐neuronal synapses, and its internalization is mediated by heavy chain

FEBS Letters

Tauc, Ladislav²  Mochida, Sumiko²  Weller, Ulrich¹  Habermann, Ernst¹  Poulain, Bernard² 

[1] Rudolf-Buchheim-Institut für Pharmakologie der Justus-Liebig-Universität, D-6300 Gießen, FRG;Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France

关键词: Tetanus toxin;    Light chain;    Heavy chain;    Transmitter release;    Central synapse;    (Aplysia);   

DOI  :  10.1016/0014-5793(89)80926-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of the two-chain form of tetanus toxin (TeTx), its constituent light (LC) or heavy (HC) chains, and papain fragment to block evoked acetylcholine (ACh) release in the buccal ganglia of Aplysia californica was studied electrophysiologically. Extracellularly applied, TeTx or its B fragment (consisting of LC and β₂, the amino-terminal portion of HC) blocked ACh release, whereas LC, HC, or the β₂ fragment did not affect it. Toxicity was restored when LC was bath applied together with HC or the β₂ fragment. When injected into the presynaptic neuron, TeTx, the B fragment or LC, but not HC, induced inhibition of ACh release. These results indicate that the blockade of ACh release by TeTx is mimicked by intracellular action of LC, the internalization of which is mediated by the HC via its amino-terminal moiety.

【 授权许可】

Unknown