Sphingolipid transport from the trans‐Golgi network to the apical surface in permeabilized MDCK cells

FEBS Letters

Bhakdi, Sucharit¹  Kobayashi, Toshihide²  Pimplikar, Sanjay W.²  Simons, Kai²  Parton, Robert G.² 

[1] Institute of Medical Microbiology, University of Mainz, Augustusplatz, 6500 Mainz, Germany;European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, Germany

关键词: Sphingolipid transport;    Fluorescent lipid analog;    Streptolysin O;    Trans Golgi network;    Permeabilized cell;    Apical membrane;    MDCK cell;    BSA;    Bovine serum albumin;    C6-NBD-Cer;    N-(7-nitrobenz-2-oxa-1;    3-diazol-4-yl)-6-aminocaproyl d-erythro-sphingosine;    C6-NBD-GleCer;    N-(7-nitrobenz-2-oxa-1;    3-diazol-4-yl)-6-aminocaproyl sphingosine glucoside;    C6-NBD-SM;    N-(7-nitrobenz-2-oxa-1;    3-diazol-4-yl)-6-aminocaproyl sphingosine-1-phosphocholine;    HA;    Hemagglutinin;    LDH;    Lactate dehydrogenase;    MDCK;    Madin Darby canine kidney;    SLO;    Streptolysin O;    TGN;    trans-Golgi network;   

DOI  :  10.1016/0014-5793(92)80851-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.

【 授权许可】

Unknown