Lipid diffusion in the thylakoid membranes of the cyanobacterium Synechococcus sp.: effect of fatty acid desaturation

FEBS Letters

Tobin, Mark J²  Sarcina, Mary³  Mullineaux, Conrad W³  Murata, Norio¹ 

[1] National Institute of Basic Biology, Okazaki 444, Japan;CLRC Daresbury Laboratory, Daresbury, Warrington, Cheshire WA4 4AD, UK;Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK

关键词: Cyanobacterium;    Thylakoid membrane;    Photosynthesis;    Lipid;    Diffusion;    Membrane fluidity;    BODIPY;    BODIPY FL C12;    4;    4-difluoro-5;    7-dimethyl-4-bora-3a;    4a-diaza-5-indacene-3-dodecanoic acid;    FRAP;    fluorescence recovery after photobleaching;    PS II;    photosystem II;   

DOI  :  10.1016/S0014-5793(03)01031-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thylakoid membranes are crucial to photosynthesis in cyanobacteria and plants. In cyanobacteria, genetic modification of membrane lipid composition strongly influences cold tolerance and susceptibility to photoinhibition. We have used fluorescence recovery after photobleaching to measure the diffusion of a lipid-soluble fluorescent marker in cells of the cyanobacterium Synechococcus sp. PCC 7942. We have compared the wild-type strain with a transformant with an increased level of fatty acid unsaturation. The transformant showed a six-fold increase in the diffusion coefficient for the fluorescent marker at growth temperature. This is the first direct measurement of lipid diffusion in a photosynthetic membrane.

【 授权许可】

Unknown