Transbilayer organization of the main chlorophyll a/b‐protein of photosystem II of thylakoid membranes

FEBS Letters

Anderson, Jan.M.¹  Goodchild, D.J.¹ 

[1] CSIRO, Division of Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia

关键词: Chlorophyll a/b-protein;    Carboxyl-terminal localization;    Immunogold labeling;    Light-harvesting complex;    Photosynthesis;    Thylakoid membrane;    Chl;    chlorophyll;    LHCII;    light-harvesting chlorophyll a/b-protein complex of PS II;    PS;    photosystem;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(87)81459-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To probe the location of the carboxyl-terminus of the 28 kDa apoprotein of the light-harvesting chlorophyll a/b-protein complex of PS II (LHCII), an antibody was generated against a synthetic octapeptide corresponding to the C-terminal region of LHCII. The high specificity of the octapeptide antiserum was deonstrated by immunoblots and immunogold labelling. The octapeptide antiserum agglutinated destacked thylakoid membranes, but no significant agglutination occurred with inside-out vesicles suggesting that the COOH-terminus is located at the outer, stroma-exposed surface where the NH₂-terminus is also located [(1983) J. Biol. Chem. 258, 9941-9948]. Our results support a model for LHCII with four transmembrane-spanning domains.

【 授权许可】

Unknown