Distribution of intrinsic and extrinsic subunits of the PS II protein complex between appressed and non‐appressed regions of the thylakoid membrane: an immunocytochemical study

FEBS Letters

Olive, J.¹  Vallon, O.¹  Wollman, F.A.² 

[1] Laboratoire de Microscopie Electronique, Institut Jacques Monod du CNRS, Université Paris VII, 2 Place Jussieu, 75251 Paris Cedex 05, France;Institut de Biologie Physico-Chimique, 13 Rue Pierre et Marie Curie, 75231 Paris Cedex 05, France

关键词: Photosystem II subunit;    Oxygen evolution site;    Membrane lateral heterogeneity;    Immunogold labeling;   

DOI  :  10.1016/0014-5793(85)80786-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The distribution of photosystem (PS) II centers between appressed and non-appressed domains of the thylakoid membranes has been investigated, using immunogold labeling, in C. reinhardtii and in spinach. We have used antibodies directed against the two main intrinsic subunits of the PS II center and against two extrinsic subunits of the O₂-evolving site. 90% of the two intrinsic PS II antigens are located in the appressed membranes, in a density 3–3.5-fold higher than in the non-appressed regions. The same distribution is observed for the two extrinsic antigens. This result suggests that the O₂-evolving system is associated with the PS II center, both in appressed and in non-appressed regions of the thylakoid membrane.

【 授权许可】

Unknown