Correlation of death modes of photosensitized cells with intracellular ATP concentration

FEBS Letters

Sadauskaite, Ausra¹  Juodka, Benediktas¹  Kirveliene, Vida¹  Sasnauskiene, Sofija¹  Kadziauskas, Jurgis¹ 

[1]Department of Biochemistry and Biophysics, Vilnius University, Ciurlionio 21, LT-2009 Vilnius, Lithuania

关键词: Apoptosis;    Photosensitization;    ATP;    Mitochondrion;    Glycolysis;    ALA;    5-aminolevulinic acid;    Ant;    antimycin A;    DG;    2-deoxyglucose;    DMEM;    Dulbecco's modified Eagle's medium;    DPBS;    Dulbecco's phosphate-buffered saline;    EP;    endogenous porphyrins;    FCS;    fetal calf serum;    m-THPC;    5;    10;    15;    20-tetrakis(m-hydroxyphenyl)-chlorine;    ROS;    reactive oxygen species;    SRB;    sulforhodamine B;    TPPS4;    meso-tetra(4-sulfonatophenyl)-porphine;   

DOI  :  10.1016/S0014-5793(03)01014-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The impact of intensity of glycolysis and oxidative phosphorylation on death of photosensitized murine hepatoma MH22 cells in vitro has been investigated. Cells photosensitized with meso-tetra(4-sulfonatophenyl)-porphine localized to lysosomes died mostly by necrosis, and the mode of cell death did not depend on the energy metabolism. Photosensitization with 5-aminolevulinic acid-stimulated endogenous porphyrins localized mainly in mitochondria or 5,10,15,20-tetrakis(m-hydroxyphenyl)-chlorine localized to cell membranes, including mitochondria, led to cell death mostly by apoptosis. In this case, the mode of cell death depended on the medium: under conditions unfavorable to glycolysis the ratio apoptosis/necrosis decreased significantly.

【 授权许可】

Unknown