Sequence of Escherichia coli O128 antigen biosynthesis cluster and functional identification of an α‐1,2‐fucosyltransferase

FEBS Letters

Shao, Jun¹  Li, Mei¹  Wang, Peng George¹  Jia, Qiang¹  Lu, Yuquan¹ 

[1] Department of Chemistry, Wayne State University, Detroit, MI 48202, USA

关键词: Lipopolysaccharide;    α (1→2) fucosyltransferase;    Escherichia coli O128 gene cluster;    EPEC;    enteropathogenic Escherichia coli;    LPS;    lipopolysaccharide;    undP;    undecaprenyl phosphate;    GalNAc;    N-acetylgalactosamine;    Gal;    galactose;    Fuc;    fucose;    PCR;    polymerase chain reaction;    GST;    glutathione S-transferase;   

DOI  :  10.1016/S0014-5793(03)00980-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

O128 is one of the most common atypical enteropathogenic Escherichia coli isolated from diarrhea patients worldwide. The primary structure of E. coli O128 repeat units has previously been determined as →3)-β-D-GalNAc-(1→4)-α-D-Gal-(1→3)-β-D-GalNAc-(1→6)-[α-L-Fuc-(1→2)]-β-D-Gal-(1→ pentasaccharide. Here we report the complete sequencing of E. coli O128 antigen biosynthesis gene cluster and its flanking regions. Comparative sequence analysis revealed the expected O128 antigen process genes, GDP-fucose biosynthesis genes and four potential glycosyltransferase genes responsible for the assembly of E. coli O128 antigen repeats. WbsJ was shown to encode an α-1,2-fucosyltransferase by enzymatic assays and nuclear magnetic resonance spectroscopy analysis.

【 授权许可】

Unknown