Localized, diffuse, and aggregative-adhering Escherichia coli from infants with acute diarrhea and matched-controls

Memórias do Instituto Oswaldo Cruz

Marcelo Magalhães¹  Rosemary J. M. Amorim¹  Yoshifumi Takeda¹  Teizo Tsukamoto¹  Maria G. Antas¹  Seiki Tateno¹ 

[1] ,Universidade Federal de Pernambuco Laboratório de Imunopatologia keizo Asami Recife,Brasil

关键词: adherent Escherichia coli;    EPEC;    infantile diarrhea;   

DOI  :  10.1590/S0074-02761992000100015

来源: SciELO

【 摘 要 】

Of 126 infants under 2 years, enrolled in a study on the etiology of acute diarrhea in Recife, Brazil, we selected 37 from whom no recognized enteropathogens, except classic enteropathogenic Escherichia coli, were identified. For comparison, we also examined 37 matched-control infants without diarrhea seen at the same hospital setting. This paper had the purpose to determine the prevalence of localized, diffuse, and aggregative-adhering E. coli strains in both groups. Three to five fecal E. coli colonies, of each case and control, were tested individually for adherence to HeLa cells by using the one step 3-h incubation assay. Strains of E. coli showing localized adherence were found significantly more often in patients (37.8%) than in controls (13.5%), p < 0.02, and they were pratically confined to EPEC serovars 055:H-, 0111:H2, and 119:H6. In contrast, E. coli isolates exhibiting the diffuse or aggregative patterns of adherence were restricted to non-EPEC serogroups and were more frequently encountred among controls.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License