Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis

Brazilian Journal of Microbiology

Paula Célia Mariko Koga²  Caroline Anunciação Menezes²  Flávia Afonso Lima²  Júlia Mitico Nara²  Caroline Arantes Magalhães²  Aurora Marques Cianciarullo¹  Jorge Mario Da Costa Ferreira-júnior¹  Luiz Rachid Trabulsi²  Meire Roberta Bresciani Mendes-ledesma²  Roxane Maria Fontes Piazza² 

[1] ,Laboratório Especial de MicrobiologiaSão Paulo SP ,Brasil

关键词: intimin;    phenotypical analysis;    polyclonal antibody;    EPEC;    EHEC;    intimina;    análise fenotípica;    anticorpo policlonal;    EPEC;    EHEC;   

DOI  :  10.1590/S1517-83822003000500002

来源: SciELO

【 摘 要 】

Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.

【 授权许可】

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