Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish

FEBS Letters

Olasz, Ferenc³  Kiss, János³  Szabó, Mónika³  Balduf, Carolin¹  Strähle, Uwe²  Müller, Ferenc¹ 

[1] Institute of Toxicology and Genetics, Forschungszentrum, Karlsruhe, Postfach 3640, D-76021 Karlsruhe, Germany;Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 10142, 67404 Illkirch Cedex, C.U. de Strasbourg, France;Environmental Biosafety Research Institute, Agricultural Biotechnology Center, Szent-Györgyi Albert St. 4, H-2101 Gödöllő, Hungary

关键词: IS30 transposase;    Gli1;    λ cI repressor;    Protein fusion;    Zebrafish;    Site-directed integration;    DBD;    DNA binding domain;    GOHS;    oligonucleotide hot spot based on E. coli genomic IS30 insertion sites;    IS;    insertion sequence;    IR;    inverted repeat;    MT;    Myc-epitope tag;    NLS;    nuclear localization signal;   

DOI  :  10.1016/S0014-5793(03)00814-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We provide evidence that a prokaryotic insertion sequence (IS) element is active in a vertebrate system. The transposase of Escherichia coli element IS30 catalyzes both excision and integration in extrachromosomal DNA in zebrafish embryos. The transposase has a pronounced target preference, which is shown to be modified by fusing the enzyme to unrelated DNA binding proteins. Joining the transposase to the cI repressor of phage λ causes transposition primarily into the vicinity of the λ operator in E. coli, and linking to the DNA binding domain of Gli1 also directs the recombination activity of transposase near to the Gli1 binding site in zebrafish. Our results demonstrate the possibility of fusion transposases to acquire novel target specificity in both prokaryotes and eukaryotes.

【 授权许可】

Unknown