Alternative splicing regulates the nuclear or cytoplasmic localization of dystrophin Dp71

FEBS Letters

Garcı́a-Sierra, Francisco³  Ray, Peter N.⁴  González, Everardo¹  Howard, Perry L.⁴  Mornet, Dominique²  Cisneros, Bulmaro¹  Montañez, Cecilia¹ 

[1] Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, Avenida Instituto Politécnico Nacional 2508, Apartado Postal 14-740, C.P. 07000, México D.F., Mexico;INSERM U 128, Institut Bouisson-Bertrand, 778 rue de la Croix Verte, 34196 Montpellier Cedex 5, France;Department of Cell and Molecular Biology, Northwestern University Medical School, Tarry 8-754, 303 Chicago Ave., Chicago, IL 60611, USA;Department of Genetics, Pediatric Laboratory Medicine and Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ont., Canada M5G 1X8

关键词: Dp71;    Alternative splicing;    Nuclear localization;    Green fluorescent protein;    Protein fusion;    DAPC;    dystrophin-associated protein complex;    GFP;    green fluorescent protein;    NPC;    nuclear pore complex;    NLS;    nuclear localization signal;   

DOI  :  10.1016/S0014-5793(00)02044-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The subcellular distribution of Dp71 isoforms alternatively spliced for exon 71 and/or 78 was examined. The cDNA sequence of each variant was fused to the C-terminus of the green fluorescent protein and the constructs were transfected transiently in the cell lines HeLa, C2C12 and N1E-115. The subcellular distribution of the fused proteins was determined by confocal microscope analysis. The Dp71 isoform lacking the amino acids encoded by exons 71 and 78 was found exclusively in the cytoplasm whereas the variants containing the amino acids encoded by exon 71 and/or exon 78 show a predominant nuclear localization. The nuclear localization of Dp71 provides a new clue towards the establishment of its cellular function.

【 授权许可】

Unknown