Functional EF‐Tu with large C‐terminal extensions in an E. coli strain with a precise deletion of both chromosomal tuf genes

FEBS Letters

Isaksson, Leif A.¹  Abdulkarim, Farhad¹  Kálmán, Miklós²  Schnell, Robert¹ 

[1] Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden;Bay Zoltán Foundation for Applied Research, Institute of Biotechnology, Szeged, Hungary

关键词: Elongation factor;    Protein fusion;    Plasmid exchange;    Gene replacement;   

DOI  :  10.1016/S0014-5793(03)00159-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An Escherichia coli strain was constructed in which both chromosomal genes encoding elongation factor (EF)-Tu (tufA and tufB) have been inactivated with precise coding sequence replacements. A tufA gene in an expression vector is supplied as the sole EF-Tu source. By using plasmid replacement, based on plasmid incompatibility, mutant EF-Tu variants with a large C′-terminal extension up to 270 amino acids were studied and proved to be functional in a strain lacking the chromosomal tufA and tufB genes.

【 授权许可】

Unknown