Mutagenesis of the gene encoding amicyanin of Paracoccus denitrificans and the resultant effect on methylamine oxidation

FEBS Letters

Oltmann, L.Fred¹  van Spanning, Rob J.M.¹  Reijnders, Willem N.M.¹  Stouthamer, Adrian H.¹  Wansell, Corry W.¹ 

[1] Department of Microbiology, Biological Laboratory, Vrije Universitat de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: Amicyanin;    Methylamine dehydrogenase;    Gene replacement;    Electron transport chain;    Paracoccus denitrificans;   

DOI  :  10.1016/0014-5793(90)81475-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of Pracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylmine dehydrogenase. Amicyanin is transcribed as a presursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine.

【 授权许可】

Unknown