Association of Caldendrin splice isoforms with secretory vesicles in neurohypophyseal axons and the pituitary

FEBS Letters

Böckers, Tobias M.⁴  Landwehr, Marco¹  Gundelfinger, Eckart D.¹  Richter, Karin²  Redecker, Peter³  Kreutz, Michael R.¹  Dieterich, Daniela C.¹ 

[1] AG Molecular Mechanisms of Plasticity, Department of Neurochemistry/Molecular Biology, Leibniz Institute for Neurobiology, Brenneckestr. 6, 39118 Magdeburg, Germany;Institute for Medical Neurobiology, Otto-von-Guericke University, Magdeburg, Germany;Department of Anatomy 1, Medical School of Hannover, Hannover, Germany;AG Molecular Neurobiology, Institute for Anatomy, Westfälische-Wilhelms University, Münster, Germany

关键词: EF-hand;    Immunogold electron microscopy;    Alternative splicing;    Hormone release;    Meuronal calcium sensor;   

DOI  :  10.1016/S0014-5793(03)00713-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Caldendrin is a neuronal calcium-binding protein, which is highly enriched in the postsynaptic density fraction and exhibits a prominent somato-dendritic distribution in brain. Two additional splice variants derive from the caldendrin gene, which have unrelated N-termini and were previously only detected in the retina. We now show that these isoforms are present in neurohypophyseal axons and on secretory granules of endocrine cells. In light of the described interaction of the Caldendrin C-terminus with Q-type Ca_v2.1 calcium channels these data suggest that this interaction takes place in neurohypophyseal axons and pituitary cells indicating functions of the short splice variants in triggering Ca²⁺ transients to a vesicular target interaction.

【 授权许可】

Unknown