High‐resolution immunogold cytochemistry indicates that AQP4 is concentrated along the basal membrane of parietal cell in rat stomach

FEBS Letters

Nagelhus, Erlend A³  Horio, Yoshiyuki¹  Kurachi, Yoshihisa¹  Ottersen, Ole Petter³  Hata, Fumiaki⁴  Fujita, Akikazu¹  Nielsen, Søren² 

[1] Department of Pharmacology II, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan;Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus, Denmark;Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, N-0317 Oslo, Norway;Department of Veterinary Pharmacology, College of Agriculture, Osaka Prefecture University, 1-1 Gakuenchou, Sakai, Osaka 599-8531, Japan

关键词: Aquaporin;    Gastric parietal cell;    Gastric chief cell;    Basal membrane;    Immunohistochemistry;    Immunogold electron microscopy;   

DOI  :  10.1016/S0014-5793(99)01256-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gastric parietal cells secrete hydrochloric acid in stomach. Because the secreted HCl solution is isotonic with the plasma fluid, it should accompany the water transport across the membranes of parietal cells. Aquaporins (AQPs) are water channel proteins that play the central role in the cellular handling of water in various mammalian tissues. Using immunocytochemistry, we found that AQP4 was expressed only in parietal cells of rat gastric mucosa. Immunogold electron microscopy study further demonstrated that AQP4 was mostly localized at the basal membrane of parietal cells. In the basal membrane, AQP4 was prominently enriched on the portion contacting with the basement membrane surrounding gastric glands. These results suggest that the contact between basement membrane and basal membrane may generate the signal involved in the targeting of AQP4 in gastric parietal cells.

【 授权许可】

Unknown