The initial phase of GnRH‐stimulated LH release from pituitary cells is independent of calcium entry through voltage‐gated channels

FEBS Letters

Wakefield, I.¹  Naor, Z.²  King, Judy A.¹  Davidson, J.S.¹  Millar, R.P.¹  Smith, Colleen E.¹ 

[1] MRC Regulatory Peptides Research Unit, Department of Chemical Pathology, UCT Medical School, Cape Town, Republic of South Africa;Department of Hormone Research, Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Kinetics;    Gonadotropin-releasing hormone;    Luteinizing hormone;    Ca2+ channel;    Ca2+ influx;    Hormone release;    (Pituitary cell);   

DOI  :  10.1016/0014-5793(87)81167-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Kinetic studies on gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone (LH) release were undertaken using rat and chicken pituitary cell cultures. In response to continuous GnRH stimulation, a biphasic pattern of LH release was demonstrated. The two phases showed different susceptibility to the voltage-gated Ca²⁺ channel blockers D600 and nifedipine. The first (transient) phase of LH release was unaffected by the Ca²⁺ channel blockers whereas the second (sustained) phase was inhibited by both drugs. These results indicate that the initial phase of LH release is independent of Ca²⁺ entry through voltage-gated Ca²⁺ channels and may depend on mobilisation of intracellular Ca²⁺ or entry of extracellular Ca²⁺ through another mechanism.

【 授权许可】

Unknown