Environmental stresses inhibit and stimulate different protein import pathways in plant mitochondria

FEBS Letters

Millar, A.Harvey¹  Taylor, Nicolas L.¹  Day, David A.¹  Rudhe, Charlotta²  Hulett, Joanne M.³  Glaser, Elzbieta²  Lithgow, Trevor³  Whelan, James¹ 

[1] Plant Molecular Biology Group, Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia;Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden;Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Vic. 3010, Australia

关键词: Environmental stress;    Mitochondrial import;    Processing;    Import pathway;    AOS;    active oxygen species;    AOX;    alternative oxidase;    FAd;    FAd subunit of mitochondrial ATP synthase;    ANT;    adenine nucleotide transporter;    SSU;    small subunit of ribulose 1;    5-bisphosphate carboxylase/oxygenase;    GR;    glutathione reductase;    RSP10;    nuclear encoded ribosomal protein 10;    Tim23;    translocase of the inner membrane protein 23;   

DOI  :  10.1016/S0014-5793(03)00691-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The impact of various environmental stresses (drought, chilling or herbicide treatment) on the capacity of plant mitochondria to import precursor proteins was investigated. Drought treatment stimulated import and processing of various precursor proteins via the general import pathway. The stimulatory effect of drought on the general import pathway was due to an increased rate of import, was accompanied by an increased rate of processing, and could be attributed to the presequence of the precursor protein. Interestingly, drought decreased the import of the F_Ad subunit of ATP synthase suggesting a bypass of the point of stimulation during import of this precursor. Both chilling and herbicide treatment of plants, on the other hand, caused inhibition of import with all precursors tested. No decrease in processing of imported proteins was observed by these stress treatments. Western analysis of several mitochondrial proteins indicated that the steady-state level of several mitochondrial components, including the TOM20 receptor and the core subunits of the cytochrome bc ₁ complex responsible for processing, remained largely unchanged. Thus environmental stresses differentially affect import of precursor proteins in a complicated manner dependent on the import pathway utilised.

【 授权许可】

Unknown