Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi)

FEBS Letters

Nieth, Christiane¹  Stege, Alexandra¹  Priebsch, Axel¹  Lage, Hermann¹ 

[1] Humboldt University Berlin, Charité Campus Mitte, Institute of Pathology, Schumannstr. 20/21, D-10117 Berlin, Germany

关键词: P-glycoprotein;    MDR1;    Gastric cancer;    Pancreatic cancer;    Gene therapy;   

DOI  :  10.1016/S0014-5793(03)00523-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For reversal of MDR1 gene-dependent multidrug resistance (MDR), two small interfering RNA (siRNA) constructs were designed to inhibit MDR1 expression by RNA interference. SiRNA duplexes were used to treat human pancreatic carcinoma (EPP85-181RDB) and gastric carcinoma (EPG85-257RDB) cells. In both cellular systems, siRNAs could specifically inhibit MDR1 expression up to 91% at the mRNA and protein levels. Resistance against daunorubicin was decreased to 89% (EPP85-181RDB) or 58% (EPG85-257RDB). The data indicate that this approach may be applicable to cancer patients as a specific means to reverse tumors with a P-glycoprotein-dependent MDR phenotype back to a drug-sensitive one.

【 授权许可】

Unknown