Expression of the MDR1 Gene and P-Glycoprotein in Canine Mast Cell Tumor Cell Lines

Journal of Veterinary Medical Science

Masaru OKUDA¹  Tomoko TAKAHASHI⁶  Tsuyoshi KADOSAWA⁴  Kazuhito ITAMOTO⁵  Nobuo SASAKI²  Yoko TAKESHITA⁵  Satoshi UNE³  Yasuho TAURA⁵  Yuya NAKAMOTO⁵  Munekazu NAKAICHI⁵ 

[1] Department of Veterinary Internal Medicine, Yamaguchi University;Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Department of Veterinary Hospital, Yamaguchi University;Department of Companion Animal Clinical Science, School of Veterinary Medicine, Rakuno Gakuen University;Department of Veterinary Surgery, Yamaguchi University;Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

关键词: canine mast cell tumor;    cellular chemosensitivity;    MDR1;    P-glycoprotein;   

DOI  :  10.1292/jvms.69.111

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(6)Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors.

【 授权许可】

Unknown