γ‐MSH increases intracellular cAMP accumulation and GnRH release in vitro and LH release in vivo

FEBS Letters

Davies, S.²  Gardiner, J.V.²  Smith, D.M.¹  Bloom, S.R.²  Stanley, S.A.²  Small, C.J.²  Ghatei, M.A.² 

[1] AstraZeneca, CVGI, Alderley Park, Macclesfield, Cheshire, UK;Endocrine Unit, Division of Investigative Science, ICSM, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK

关键词: Melanocortin;    Gamma melanocyte-stimulating hormone;    Alpha melanocyte-stimulating hormone;    Gonadotropin-releasing hormone;    Hypothalamus;   

DOI  :  10.1016/S0014-5793(03)00402-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The roles of the melanocortin 3 receptor (MC3-R) and its agonist, γ₂-melanocyte-stimulating hormone (γ₂-MSH) in the regulation of the hypothalamo-pituitary-gonadal (HPG) axis are poorly understood. Here we show γ₂-MSH stimulated intracellular cAMP accumulation and gonadotrophin-releasing hormone (GnRH) secretion in the immortalised GnRH cell line GT₁-7. The MC3/4-R antagonist Agrp blocked these actions. Reverse transcriptase polymerase chain reaction demonstrated GT₁-7 cells express MC3-R mRNA. γ₂-MSH also stimulated GnRH release from hypothalamic explants. In vivo, γ₂-MSH administration into the medial preoptic area significantly increased plasma luteinising hormone. MC3-R and γ₂-MSH may modulate the HPG axis.

【 授权许可】

Unknown