Solubilisation partial characterisation of the α‐MSH receptor on primary rat Schwann cells

FEBS Letters

Ahmed, Abdel R.H.²  Oliver, George W.J.²  Dyer, Jason K.¹  Haynes, Laurence W.¹  Poulton, Colin W.² 

[1] Department of Zoology, School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK;School of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath BA2 7AY, UK

关键词: α-Melanocyte stimulating hormone;    Receptor isolation;    Melanocortin;    Schwann cell;    Nerve regeneration;   

DOI  :  10.1016/0014-5793(93)81619-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ACTH/MSH_4–10 melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent α-MSH analogue to solubilise α-MSH receptor proteins from cultured primary rat Schwann cells. [¹²⁵I-Tyr²,Nle⁴,d-Phe⁷,ATB-Lys¹¹]-α-MSH photoaffinity labelled proteins from Schwann cells were analyzed by SDS-PAGE followed by autoradiography. The results indicate that the α-MSH receptor proteins labelled have a molecular weight of 42–45 kDa. These data are the first to demonstrate solubilisation and characterisation of α-MSH receptors from non-melanoma cells.

【 授权许可】

Unknown