A new ratiometric fluorescence probe as strong sensor of surface charge of lipid vesicles and micelles

FEBS Letters

Bhattacharya, Santanu¹  Singh, Yashveer¹  Gulyani, Akash¹ 

[1] Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India

关键词: Membrane surface charge;    Ratiometric probe;    Pyren-1-yl-vinyl pyridine;    Anionic surface;    Phospholipid vesicle;    Micelle;    CTAB;    cetyl trimethyl ammonium bromide;    Tween® 20;    polyoxyethylene (20) sorbitan monolaureate;    SDS;    sodium dodecyl sulfate;    DPPC;    dipalmitoylphosphatidyl choline;    DCP;    dicetyl phosphate;    DPPA;    dipalmitoylphosphatidic acid;    CT;    charge transfer;   

DOI  :  10.1016/S0014-5793(03)00314-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report on the ability of a new fluorescent probe, 4-(2-pyren-1-yl-vinyl) pyridine, 1, to respond to micelles and phospholipid vesicles of different surface charge. The probe gets incorporated into micellar and membranous assemblies and shows a large red-shift in the fluorescence emission maxima especially when the surface charge of the organized media is anionic. The effect on the photo-excitation of the probe is very clear and pronounced as it can be easily visualized. The sample color upon photo-excitation changes from blue to orange/red once the probe experiences negatively charged vesicular or micellar surfaces. These results make the probe molecule useful as a reporter for sensing electrostatic environment in biological membranes and related organized assemblies.

【 授权许可】

Unknown