Neutral fluorescence probe with strong ratiometric response to surface charge of phospholipid membranes

FEBS Letters

Demchenko, Alexander²  Klymchenko, Andrey²  Duportail, Guy¹  Mely, Yves¹ 

[1] Laboratoire de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculté de Pharmacie, Université Louis Pasteur, BP 24, 67401 Illkirch, France;A.V. Paladin Institute of Biochemistry, 9 Leontovicha str., 01030 Kiev, Ukraine

关键词: Membrane surface charge;    Fluorescent ratiometric probe;    Phospholipid vesicle;    3-Hydroxyflavone;    Excited state proton transfer;    CTAB;    cetyl trimethylammonium bromide;    PG;    phosphatidyl glycerol;    PC;    phosphatidylcholine;    EYPC;    egg yolk PC;    EYPG;    egg yolk PG;    DMTAP;    N-[1-(2;    3-dimyristoyloxy)propyl]-N;    N;    N-trimethylammonium tosylate salt;   

DOI  :  10.1016/S0014-5793(01)03055-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report on dramatic differences in fluorescence spectra of 4′-dimethylamino-3-hydroxyflavone (probe F) studied in phospholipid membranes of different charge (phosphatidyl glycerol, phosphatidylcholine (PC), their mixture and the mixture of PC with a cationic lipid). The effect consists in variations of relative intensities at two well-separated band maxima at 520 and 570 nm belonging to normal (N*) and tautomer (T*) excited states of flavone chromophore. Based on these studies we propose a new approach to measure electrostatic potential at the surface layer of phospholipid membranes, which is based on potential-dependent changes of bilayer hydration and involves very sensitive and convenient ratiometric measurements in fluorescence emission.

【 授权许可】

Unknown