Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design

FEBS Letters

Garcı́a, Mónica¹  Haro, Isabel¹  Chan, Weng C³  Pérez, Silvia¹  Ercilla, Guadalupe² 

[1] Departament de Quı́mica de Pèptids i Proteı̈nes, IIQAB-CSIC, Jordi Girona 18–26, 08034 Barcelona, Spain;Servei d’Immunologia, IDIBAPS, Hospital Clı́nic i Provincial, Barcelona, Spain;School of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK

关键词: Hepatitis A virus;    Multiple antigenic peptide;    Peptide synthesis;    Liposome;    Enzyme-linked immunosorbent assay;    Surface plasmon resonance;    Boc;    tert-butoxycarbonyl;    DIEA;    N;    N-diisopropylethylamine;    DIPCDI;    N;    N-diisopropylcarbodiimide;    DMF;    dimethylformamide;    ELISA;    enzyme-linked immunosorbent assay;    Fmoc;    fluoren-9-ylmethoxycarbonyl;    HAV;    hepatitis A virus;    HOBt;    N-hydroxybenzotriazole;    HPLC;    high-performance liquid chromatography;    MAP;    multiple antigenic peptides;    MLV;    multilamellar vesicles;    MS;    mass spectrometry;    OD;    optical density;    RU;    resonance units;    SPR;    surface plasmon resonance;    TBTU;    2(1H-benzotriazol-1-yl)-1;    1;    3;    3-tetramethyluronium tetrafluoroborate;    TFA;    trifluoroacetic acid;    TIPS;    triisopropylsilane;   

DOI  :  10.1016/S0014-5793(03)00249-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Multiple antigen peptides (MAP) have been demonstrated to be efficient immunological reagents for the induction of immune responses to a variety of infectious agents. Several peptide domains of the hepatitis A virus (HAV) capsid proteins, mainly VP1 and VP3, are the immunodominant targets for a protective antibody response. In the present study we analyse the immunogenic properties of a tetrameric heterogeneous palmitoyl-derivatised MAP containing two defined HAV peptide sequences, VP1(11–25) and VP3(102–121), in rabbits immunised with either Freund's adjuvant or multilamellar liposomes. The immune response was evaluated with a specific enzyme immunoassay using MAP[VP1+VP3], VP1 and VP3 as targets. The avidity of the immune response was measured by a non-competitive enzyme-linked immunosorbent assay and by the surface plasmon resonance technology. Antisera raised against the lipo-MAP peptide entrapped in liposomes demonstrated high avidity of binding with affinity rate constants approximately one order of magnitude greater than those obtained with the Freund's protocol.

【 授权许可】

Unknown