Enhancement of the immunogenicity of a synthetic peptide bearing a VP3 epitope of hepatitis A virus

FEBS Letters

Garcı́a, Mónica²  Haro, Isabel²  Gómara, M.José²  Guix, Susana¹  Sánchez, Gloria¹  Bosch, Albert¹  Pintó, Rosa M¹  González-Dankaart, Juan F¹ 

[1] Department of Microbiology, University of Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain;Department of Peptides, CID, CSIC, 08034 Barcelona, Spain

关键词: Hepatitis A virus;    B-epitope;    T-epitope;    Synthetic peptide;    Liposome;    HAV;    hepatitis A virus;    MPNCU/ml;    most probable number of cytopathogenic units per ml;    HBsAg;    hepatitis B virus surface antigen;    FCA;    Freund's complete adjuvant;    ELISA;    enzyme linked immunosorbent assay;    MAP;    multiple antigenic peptide;    FMDV;    foot-and-mouth disease virus;    BSA;    bovine serum albumin;   

DOI  :  10.1016/S0014-5793(98)01278-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The immune responses elicited in mice by different forms of the VP3(110–121) B-epitope of the hepatitis A virus (HAV) were studied. Different forms of incorporation in liposomes were tested, encapsulation, rather than surface exposure, being the best antigenic preparation. Three larger peptides of the VP3 epitope, two of them containing a hepatitis B virus T-epitope, and a third containing a putative T-epitope of HAV (VP3(102–121)) were assayed. While this latter T-epitope induced an enhancement of the response against the VP3 B-epitope, the artificially coupled T-epitopes failed to induce a significant increase. The administration of two multiple antigenic peptide (MAP) constructs, the first containing the VP3(110–121) and VP1(11–25) HAV sequences and the second only the VP1(11–25) sequence, also suggested the presence of a T-epitope, since the response against the VP1 peptide was increased in the first construct.

【 授权许可】

Unknown