Osmomechanical stress selectively regulates translocation of protein kinase C isoforms

FEBS Letters

Zhang, M.I.N.¹  Liu, X.¹  O'Neil, R.G.¹  Peterson, L.B.¹ 

[1] Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, USA

关键词: Mechanical stress;    Swelling;    Hypotonic;    Protein kinase C;    Translocation;    Epithelium;   

DOI  :  10.1016/S0014-5793(03)00150-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Osmomechanical stress, resulting in cell swelling and activation/regulation of numerous cellular processes, may play a critical role in cell signaling by selectively regulating translocation of protein kinase C (PKC) isoforms from cytosol to membrane compartments. Western blotting of renal epithelial cell fractions demonstrated the expression of five PKC isoforms. Three of these isoforms (PKCα, PKCϵ, PKCζ) translocated to the membrane fraction upon exposure of cells to osmomechanical stress (hypotonic medium). Immunohistochemical staining of cells using isoform-specific antibodies further demonstrated translocation of the phorbol ester-sensitive isoforms, PKCα and PKCϵ, to both the plasma membrane and perinuclear sites, reflecting potential initial steps in regulation of specific effector pathways. Indeed, selective inhibition of PKCs indicates a potential role for PKCα in modulating a calcium influx channel. It is concluded that osmomechanical stress induces selective translocation of specific PKC isoforms, demonstrating a key role of osmomechanical stress in selectively regulating PKC-dependent signaling pathways.

【 授权许可】

Unknown