Increase of c‐jun mRNA upon hypo‐osmotic cell swelling of rat hepatoma cells

FEBS Letters

Häussinger, Dieter²  Newsome, William²  Finkenzeller, Günter²  Lang, Florian¹ 

[1] Physiologisches Institut der Universität Tübingen, Tübingen, Germany;Medizinische Universitätsklinik Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany

关键词: c-jun;    c-fos;    Proto-oncogene;    Cell swelling;    Hypotonic;    Hypertonic;    Protein kinase C;    Hepatoma cell;    PKC;    protein kinase C;    PMA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/0014-5793(94)80129-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

c-jun mRNA levels were increased in rat hepatoma cells (H4-II-E-C3) when exposed to hypotonie medium (205 ) with a maximal induction observed after 1 h of hypotonie exposure. At this time point an approximate 5-fold increase in c-jun expression could be detected in relation to nonnotonic control incubations (305 ). Hypertonic exposure (405 mosmol/1) had only a slight effect on c-jun expression. In contrast to the increased c-jun mRNA levels under hypotonic conditions, expression of the c-fos proto-oncogene was unaffected by changes in the osmolarity. The hypotonicity-induced increase in c-jun expression was also detectable in the presence of a protein kinase C (PKC) inhibitor. This indicates that PKC is not involved in the signal transduction pathway leading to c-jun expression upon hypotonic cell swelling in these cells.

【 授权许可】

Unknown