Myeloperoxidase/nitrite‐mediated lipid peroxidation of low‐density lipoprotein as modulated by flavonoids

FEBS Letters

Sies, Helmut¹  Schewe, Tankred¹  Kostyuk, Vladimir A.¹  Kraemer, Tilo¹ 

[1] Institut für Physiologische Chemie I, Universitätsklinikum Düsseldorf, Heinrich-Heine-Universität, P.O. Box 10 10 07, 40001 Düsseldorf, Germany

关键词: Atherosclerosis;    Flavanol;    Flavonol;    Oxidized LDL;    Peroxidase;    Reactive nitrogen species;    GOD;    glucose oxidase;    LDL;    low-density lipoprotein;    MPO;    myeloperoxidase;   

DOI  :  10.1016/S0014-5793(03)00113-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the presence of a H₂O₂-generating system, myeloperoxidase (MPO) caused conjugated diene formation in low-density lipoprotein (LDL), indicating lipid peroxidation which was dependent on nitrite but not on chloride. The oxidation of LDL was inhibited by micromolar concentrations of flavonoids such as (−)-epicatechin, quercetin, rutin, taxifolin and luteolin, presumably via scavenging of the MPO-derived NO₂ radical. The flavonoids served as substrates of MPO leading to products with distinct absorbance spectra. The MPO-catalyzed oxidation of flavonoids was accelerated in the presence of nitrite.

【 授权许可】

Unknown