Interaction of NF‐Y with the 3′‐flanking DNA sequence of the CCAAT box

FEBS Letters

Takishima, Kunio¹  Sugiura, Naoaki¹ 

[1] Department of Biochemistry I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan

关键词: Transcription factor;    NF-Y;    CCAAT box;    DNA binding;    CBF;    CCAAT-binding factor;    DBD;    DNA-binding domains;    CHO;    Chinese hamster ovary;    EMSA;    electrophoretic mobility shift assay;    ERK;    extracellular signal-regulated kinase;   

DOI  :  10.1016/S0014-5793(03)00071-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NF-Y, also referred to as CCAAT-binding factor, is a major CCAAT-binding transcription factor. The present study demonstrated that the 3′-flanking region of the CCAAT box is involved in the formation of a stable NF-Y·DNA complex. An electrophoretic mobility shift assay showed that the interaction of NF-Y with DNA 15 bp downstream of the CCAAT box alters not only the affinity of NF-Y for its binding site but also the electrophoretic mobility of the NF-Y·DNA complex. This interaction is accompanied by a conformational change of NF-Y as demonstrated by a change in the reactivity of an anti-NF-YA antibody to the NF-Y·DNA complex.

【 授权许可】

Unknown