Identification of proteins that interact with NF‐YA

FEBS Letters

Yamada, Kazuya¹  Granner, Daryl K.²  Osawa, Haruhiko² 

[1] Department of Biochemistry, Fukui Medical University, Fukui 910-1193, Japan;Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 707 Light Hall, Nashville, TN 37232-0615, USA

关键词: Transcription factor;    Nuclear factor-Y;    Serum response factor;    Zinc-finger;    Homeobox 1;    Protein-protein interaction;    NF-Y;    nuclear factor-Y;    NF-YA;    A subunit of NF-Y;    AD;    activation domain;    ZHX1;    zinc-fingers and homeobox-1;    SRF;    serum response factor;    Znf;    zinc-fingers;    HD;    homeodomain;    GST;    glutathione S-transferase;    PCR;    polymerase chain reaction;    DBD;    DNA binding domain;    SID;    subunit interaction domain;    SD-His;    histidine-free synthetic dextrose;    HNF4;    hepatocyte nuclear factor 4;   

DOI  :  10.1016/S0014-5793(99)01311-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHX1) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.

【 授权许可】

Unknown