Involvement of NF‐Y and Sp1 binding sequences in basal transcription of the human telomerase RNA gene

FEBS Letters

Keith, W.Nicol¹  Zhao, Jiangqin¹  Hoare, Stacey F.¹  Bilsland, Alan¹ 

[1] Cancer Research UK Department of Medical Oncology, University of Glasgow, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK

关键词: Telomerase;    hTR;    Gene promoter;    Gene transcription;    Chromatin immunoprecipitation;    NF-Y;    SPI;    EMSA;    electrophoretic mobility shift assay;    NF-Y;    nuclear factor-Y;    hTR;    human telomerase RNA gene;    ChIP;    chromatin immunoprecipitation;   

DOI  :  10.1016/S0014-5793(03)00038-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proximal promoter of the telomerase RNA gene, hTR, contains four Sp1 sites and one CCAAT box. We have carried out a functional analysis of the role of these sequence elements. Two Sp1 sites downstream of the CCAAT box mediated negative regulation, while the other two Sp1 sites were positive regulators with the strongest effect mediated by the negative regulatory Sp1 site closely flanking the CCAAT box. Basal transcriptional activity is maintained via the CCAAT box even when all four Sp1 sites are mutated, suggesting nuclear factor-Y (NF-Y) is a fundamental regulator of hTR promoter function. Chromatin immunoprecipitation revealed binding of NF-Y, Sp1 and TFIIB to the promoter in vivo. Thus the interaction of NF-Y at the CCAAT box is pivotal to hTR gene transcription and surrounding sequence elements may provide an environment for the regulation of activity through recruitment of additional protein complexes.

【 授权许可】

Unknown