Catalytic subunit of telomerase expression is related to RNA component expression

FEBS Letters

Niccolini, Benedetta¹  Raccanelli, Anna¹  Stanta, Giorgio¹  Baralle, Francisco¹  Bonin, Serena¹ 

[1] International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy

关键词: Telomerase;    Cancer;    Preneoplastic lesion;    Reverse transcription polymerase chain reaction;    Paraffin-embedded tissue;    hTR;    RNA component of human telomerase;    hTRT;    mRNA of the catalytic subunit of human telomerase;    RT-PCR;    reverse transcription PCR;    TRAP;    telomeric repeat amplification protocol;    CIN;    cervix intraepithelial neoplasia;   

DOI  :  10.1016/S0014-5793(99)01357-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Telomere length is maintained by the ribonucleoprotein enzyme telomerase. The RNA component of telomerase (hTR) is widespread, and only the expression of the mRNA encoding the catalytic protein subunit (hTRT) is correlated with telomerase activity. We have studied the level of expression of hTR and hTRT in four different models of neoplastic and preneoplastic lesions using the RT-PCR method on RNA extracted from paraffin-embedded human tissues after microdissection. The expression at the mRNA level was compared with the enzymatic activity. Our results suggest that there may be a reciprocal control at the transcriptional level of the expression of hTRT and hTR which in turn is associated with tumor progression.

【 授权许可】

Unknown