Diversity of the human erythrocyte membrane sialic acids in relation with blood groups

FEBS Letters

Bratosin, Daniela¹  Pons, Alexandre²  Bulai, Tatiana²  Montreuil, Jean²  Zanetta, Jean-Pierre² 

[1] National Institute for Biological Science Research and Development, Bucarest, Romania;Laboratoire de Glycobiologie Structurale et Fonctionnelle, CNRS Unité Mixte de Recherche No. 8576, Université des Sciences et Technologies de Lille, Bâtiment C9, 59655 Villeneuve d'Ascq Cedex, France

关键词: Sialic acid;    Red blood cell;    Blood group;    Human erythrocyte;    RBC;    red blood cells;    GC;    gas chromatography;    MS;    mass spectrometry;    HFBAA;    heptafluorobutyric anhydride;    HFB;    heptafluorobutyrate;    Kdn;    3-deoxy-D-glycero-D-galacto-nonulosonic acid;    The abbreviations of sialic acids are after Schauer and Kamerling [1];   

DOI  :  10.1016/S0014-5793(02)03838-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The composition of the human erythrocyte membrane (RBC) glycoprotein- and glycolipid-bound sialic acids of A, B, AB and O type donors was studied using a new method (Zanetta et al., Glycobiology 11 (2001) 663–676). In addition to Neu5Ac as the major compound, Kdn, Neu5,9Ac₂, Neu5,7Ac₂, Neu (de-N-acetylated-Neu5Ac), Neu5Ac8Me, Neu5Ac9Lt, Neu4,5Ac₂, Neu5,8Ac₂9Lt and Neu5Ac8S were characterised. Among these different compounds, Neu5Ac8Me, Neu5Ac9Lt, Neu4,5Ac₂, Neu5,8Ac₂9Lt and Neu5Ac8S have never been described and quantitatively determined before in human tissues or cells. Neu5Gc and its O-alkylated or O-acylated derivatives were not detected.

【 授权许可】

Unknown