Alteration of the erythrocyte glutathione redox balance by N‐acetylcysteine, captopril and exogenous glutathione

FEBS Letters

Reglinski, John¹  Abdullah, Ibrahim B.²  Smith, W.Ewen¹  McMurray, John²  Spickett, Corinne M.¹  Russell, Jim¹ 

[1] Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, UK;Cardiology Unit, Glasgow Western Infirmary, Dumbarton Road, Glasgow, G11 6NT, UK

关键词: NMR;    Antioxidant defence;    Glutathione;    N-Acetylcysteine;    Captopril;    Human erythrocyte;    BSO;    buthionine sulphoximine;    NAC;    N-acetylcysteine;   

DOI  :  10.1016/0014-5793(94)00548-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the thiol containing compounds N-acetylcysteine and captopril on glutathione metabolism in human erythrocytes has been investigated non-invasively using ¹H spin echo NMR. N-Acetylcysteine was found to increase the reduced form of glutathione while captopril increased the oxidized form, but no changes in the total glutathione concentration were observed. Incubation of the cells with buthionine sulphoximine to inhibit de novo glutathione synthesis did not affect the response. The results show that these compounds act by altering the redox balance of glutathione rather than by stimulating its synthesis, and that their mechanisms of action are different, and not simply due to the presence of the thiol group.

【 授权许可】

Unknown