Possibility of determination of the level of antioxidants in human body using spectroscopic methods

International Conference of Young Scientists and Specialists "Optics-2015"

Timofeeva, E.^1 ; Gorbunova, E.^1

ITMO University, Saint Petersburg National Research University of Information Technologies' Mechanics and Optics, Saint Petersburg, Russia^1

关键词: Anti-oxidant activities;    Antioxidant defence;    Chemiluminescent reactions;    Human bodies;    Individual components;    Multi-component systems;    Spectral composition;    Spectroscopic method;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/735/1/012035/pdf
DOI  :  10.1088/1742-6596/735/1/012035

来源: IOP

【 摘 要 】

In this work, the processes of antioxidant defence against aggressive free radicals in human body were investigated theoretically; and the existing methods of diagnosis of oxidative stress and disturbance of antioxidant activity were reviewed. Also, the kinetics of free radical reactions in the oxidation of luminol and interaction antioxidants (such as chlorophyll in the multicomponent system of plant's leaves and ubiquinone) with the UV radiation were investigated experimentally by spectroscopic method. The results showed that this method is effective for recording the luminescence of antioxidants, free radicals, chemiluminescent reactions and fluorescence. In addition these results reveal new opportunities for the study of the antioxidant activity and antioxidant balance in a multicomponent system by allocating features of the individual components in spectral composition. A creation of quality control method for drugs, that are required for oxidative stress diagnosis, is a promising direction in the development of given work.