Identification of a MAP65 isoform involved in directional expansion of plant cells

FEBS Letters

Smertenko, Andrei³  Naldrett, Mike²  Mao, Guojie¹  Bottrill, Andrew²  Hussey, Patrick J.³  Chan, Jordi¹  Lloyd, Clive W.¹ 

[1] Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK;Proteomics Facility, John Innes Centre, Norwich NR4 7UH, UK;The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Rd., Durham DH1 3LE, UK

关键词: MAP65;    Plant cytoskeleton;    Cortical microtubule;    Proteomics;    MAP;    microtubule-associated protein;    MALDI-TOF MS;    matrix-assisted laser desorption/ionisation;    time-of-flight mass spectrometry;    Q-TOF-MS;    quadrupole time-of-flight mass spectrometry;   

DOI  :  10.1016/S0014-5793(02)03848-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MAP65 comprises a multigene family specific to plants. To see which isoform is utilised for the unique mechanism of cell expansion, uncomplicated by division structures, carrot cells were deprived of auxin whereupon they stopped dividing and elongated instead. During elongation, a MAP65 protein triplet reduced to a single band. Mass spectrometric analysis demonstrated that this corresponded to a single carrot cDNA; it also corresponded to the major protein previously shown to form filamentous cross-bridges between microtubules in vitro. This MAP65 isoform is concluded to have a major role in establishing the parallel microtubule arrays characteristic of cells undergoing directional expansion.

【 授权许可】

Unknown