Gibberellic acid stabilises microtubules in maize suspension cells to cold and stimulates acetylation of α‐tubulin 1

FEBS Letters

Lloyd, Clive W¹  Huang, Rong Feng¹ 

[1] Department of Cell Biology, John Innes Centre, Colney, Norwich NR4 7UH, UK

关键词: Microtubule;    Acetylated tubulin;    Maize cell suspension (BMS);    Cold;    Plant cytoskeleton;   

DOI  :  10.1016/S0014-5793(98)01718-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gibberellic acid is known to stabilise microtubules in plant organs against depolymerisation. We have now devised a simplified cell system for studying this. Pretreatment of a maize cell suspension with gibberellic acid for just 3 h stabilised protoplast microtubules against depolymerisation on ice. In other eukaryotes, acetylation of α-tubulin is known to correlate with microtubule stabilisation but this is not established in plants. By isolating the polymeric tubulin fraction from maize cytoskeletons and immunoblotting with the antibody 6-11B-1, we have demonstrated that gibberellic acid stimulates the acetylation of α-tubulin. This is the first demonstrated link between microtubule stabilisation and tubulin acetylation in higher plants.

【 授权许可】

Unknown