Identification and characterization of single‐domain thiosulfate sulfurtransferases from Arabidopsis thaliana

FEBS Letters

Papenbrock, Jutta¹  Bauer, Michael¹ 

[1] Institute for Botany, University of Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany

关键词: Arabidopsis thaliana;    3-Mercaptopyruvate;    Protein domain;    Rhodanese;    Sulfurtransferase;    Thiosulfate;   

DOI  :  10.1016/S0014-5793(02)03723-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sulfurtransferases/rhodaneses (ST) are a group of enzymes widely distributed in all three phyla that catalyze the transfer of sulfur from a donor to a thiophilic acceptor substrate. All ST contain distinct structural domains, and can exist as single-domain proteins, as tandemly repeated modules in which the C-terminal domain bears the active site, or as members of multi-domain proteins. We identified several ST in Arabidopsis resembling the C-terminus of the Arabidopsis two-domain ST1 and the single-domain GlpE protein from Escherichia coli. Two of them (accession numbers BAB10422 and BAB10409) were expressed in E. coli and purified. Both proteins showed thiosulfate-specific ST enzyme activity.

【 授权许可】

Unknown