FEBS Letters | |
Identification and characterization of single‐domain thiosulfate sulfurtransferases from Arabidopsis thaliana | |
Papenbrock, Jutta1  Bauer, Michael1  | |
[1] Institute for Botany, University of Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany | |
关键词: Arabidopsis thaliana; 3-Mercaptopyruvate; Protein domain; Rhodanese; Sulfurtransferase; Thiosulfate; | |
DOI : 10.1016/S0014-5793(02)03723-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sulfurtransferases/rhodaneses (ST) are a group of enzymes widely distributed in all three phyla that catalyze the transfer of sulfur from a donor to a thiophilic acceptor substrate. All ST contain distinct structural domains, and can exist as single-domain proteins, as tandemly repeated modules in which the C-terminal domain bears the active site, or as members of multi-domain proteins. We identified several ST in Arabidopsis resembling the C-terminus of the Arabidopsis two-domain ST1 and the single-domain GlpE protein from Escherichia coli. Two of them (accession numbers BAB10422 and BAB10409) were expressed in E. coli and purified. Both proteins showed thiosulfate-specific ST enzyme activity.
【 授权许可】
Unknown
【 预 览 】
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